The video demonstrates haemolymph collection from adult giant freshwater prawn, Macrobrachium rosenbergii. Haemolymph was collected from healthy adult prawn. Haemolymph was withdrawn from ventral sinus, located at the base of first abdominal segment of the prawn with 10 ml syringe along with 26 gauge needle. The syringe was coated with anticoagulant (30 mM trisodium citrate, 100 mM glucose, 510 mM NaCl, 10 mM EDTA, Na , pH = 4.6) in ratio 2:1 of haemolymph. Haemolymph was then transferred to centrifuge tube and was mixed gently with anticoagulant and incubated for 5 – 10 min. Haemocytes can be collected after 5 min of centrifugation at 1800 rpm.

Reference: Chong, C.M., Yaakob, A.S. and Maha, A., 2020. Passive transfer of immunity from Tilapia antisera enhanced in-vitro phagocytosis of Aeromonas hydrophila in naïve Macrobrachium rosenbergii haemocytes. JOURNAL OF ENVIRONMENTAL BIOLOGY, 41(5), pp.1147-1152.

Further reading

Classical characteristic of the innate immune system is the lack of ability to build up immunological memory, contrast to the adaptive immune system that is capable of “remembering” antigens, and rapidly mount a greater magnitude of immune response upon subsequent exposure to the same antigens. Peculiarly, immunological memory of innate immunity is evidenced in invertebrates. At least three different memory phenomena have been described, namely sustained unique response, recalled response, and immune shift.

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